A new signal sequence trap using alkaline phosphatase as a reporter
نویسندگان
چکیده
منابع مشابه
A new signal sequence trap using alkaline phosphatase as a reporter.
Secreted and transmembrane proteins are critical to the cell-cell interactions governing normal development and carcinogenesis. To facilitate the identification of such molecules, we have developed a novel signal sequence trap that uses human placental alkaline phosphatase as a reporter. Libraries from mouse prostate and human prostatic carcinoma were constructed to test the PST (peptide signal...
متن کاملSignal sequence of alkaline phosphatase of Escherichia coli.
The amino acid sequence of the signal sequence of phoA was determined by DNA sequencing by using the dideoxy chain termination technique (Sanger et al., Proc. Natl. Acad. Sci. U.S.A. 74:5463-5467, 1977). The template used was single-stranded DNA obtained from M13 on f1 phage derivatives carrying phoA, constructed by in vitro recombination. The results confirm the sequence of the first five amin...
متن کاملLocation as a Poverty Trap
In this paper, we ask “Can proximity to towns reduces poverty in rural areas?” For this study we have mapped all the villages in Orissa. The study is based on small area poverty estimation methodology as well as the secondary data covering 47,395 villages and 109 towns. Our main findings are (i) there is a relationship between the distance to nearest town and rural poverty and (ii) poverty leve...
متن کاملAn Alkaline Phosphatase Reporter Gene Assay for Induction of CYP3A4 In Vitro
CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...
متن کاملAn Alkaline Phosphatase Reporter Gene Assay for Induction of CYP3A4 In Vitro
CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1999
ISSN: 1362-4962
DOI: 10.1093/nar/27.4.1219